Concerns about KIM-1 as a urinary biomarker for acute tubular necrosis (ATN)

Assay Validation for KIM-1: human urinary renal dysfunction biomarker

Urinary kidney injury molecule (KIM-1) is a sensitive quantitative biomarker for early detection of kidney tubular injury. The objective of the present work was to analytically validate this urinary renal injury biomarker. Duo-set reagents from R&D were used to develop the ELISA and validate the assay's linearity, intra-run precision, inter-run precision, lower limit of quantification, recovery...

Urinary kidney injury molecule-1 (Kim-1): a sensitive quantitative biomarker for early detection of kidney tubular injury

Sensitive and specific biomarkers are needed to detect early kidney injury. The objective of the present work was to develop a sensitive quantitative urinary test to identify renal injury in the rodent to facilitate early assessment of pathophysiological influence and drug toxicity. Two mouse monoclonal antibodies were made against the purified ectodomain of kidney injury molecule-1 (Kim-1) and...

Kidney Injury Molecule -1 (Kim-1): A Tissue and Urinary Biomarker for Nephrotoxicant-Induced Renal Injury

Urinary KIM-1: a novel biomarker for evaluation of occupational exposure to lead

Chronic occult lead poisoning often develops ensuing occupational lead exposure. Early diagnosis of lead poisoning is critical for timely discontinuation of lead exposure and for prognosis. This study explored the value of urinary kidney injury molecule-1 (KIM-1) in diagnosing renal injury induced by lead at an early stage. We retrospectively analyzed 92 workers exposed to occupational lead and...

Acute Tubular Necrosis

A rapid rise in creatinine following periods of hypotension is most commonly due to acute tubular necrosis. In ATN, urinalysis usually shows granular casts. Urine sodium is typically high due to tubular sodium loss, concentration is typically above 30 mmol/l. The urine to plasma osmolality ratio <1:1, the ability to concentrate urine is preserved despite sodium loss. Acute Tubular Necrosis is a...